phanta flash master mix (Vazyme Biotech Co)

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Vazyme Biotech Co phanta flash master mix
Phanta Flash Master Mix, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phanta+flash+master+mix/Phanta+Flash+Master+Mix+-+Dye+Plus/pmc13649023-149-14-20
Average 96 stars, based on 211 article reviews

phanta flash master mix - by Bioz Stars, 2026-10

96/100 stars

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Polymerase Chain Reaction:

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
Article Snippet: .. PCR amplification was performed via 2 × Phanta Flash Master Mix (Vazyme, Nanjing, China), restriction enzymes were from Takara Biomedical Technology (Beijing) Co., Ltd. .. The DNA purification kits was from Magen Biotechnology (Guangzhou) Co., Ltd., and the DNA ligation was taken by ClonExpress Ultra One Step Cloning Kit V2 (Vazyme, Nanjing, China).

Article Title: Molecular Mechanism of Multi-Tepal Flower Formation in Cymbidium ensifolium.
Article Snippet: Cymbidium ensifolium exhibits highly specialized floral structures with considerable morphological diversity, including multitepal varieties that are distinguished by unique floral patterning and are of high ornamental value, making them a key breeding target.. However, the molecular mechanisms underlying multitepal formation in this species remain poorly characterized.. To address this, we employed an integrated approach, combining phenotypic observation, cytological examination, transcriptome sequencing, gene expression profiling, functional validation, yeast onehybrid (Y1H), and dualluciferase reporter (DLR) assays.

Article Title: A membrane-free spot-plating protocol for Agrobacterium -mediated transformation of diverse yeasts
Article Snippet: .. Colony PCR for Botryozyma nematodophila and Metschnikowia pulcherrima transformants were conducted using 2 × Phanta Flash Master Mix (Dye Plus) (Vazyme Biotech, CN). ..

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
Article Snippet: .. PCR amplification was carried out using 2 × Phanta Flash Master Mix (Vazyme, Nanjing, China) with an annealing temperature of 61 °C and an extension time of 10 s. The PCR products were recovered by agarose gel electrophoresis to obtain amplicons of the BC5 library plasmids before and after screening, which were then subjected to subsequent next-generation sequencing (NGS) analysis (GENEWIZ, Suzhou, China). ..

Amplification:

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
Article Snippet: .. PCR amplification was carried out using 2 × Phanta Flash Master Mix (Vazyme, Nanjing, China) with an annealing temperature of 61 °C and an extension time of 10 s. The PCR products were recovered by agarose gel electrophoresis to obtain amplicons of the BC5 library plasmids before and after screening, which were then subjected to subsequent next-generation sequencing (NGS) analysis (GENEWIZ, Suzhou, China). ..

Article Title: Genomic characterization, recombination analysis and pathogenicity of goose astrovirus 2 isolated from geese in Jiangxi Province, China.
Article Snippet: .. The complete GoAstV-2 genome was amplified using 2 × Phanta Flash Master Mix (Vazyme, P520). ..

Agarose Gel Electrophoresis:

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
Article Snippet: .. PCR amplification was carried out using 2 × Phanta Flash Master Mix (Vazyme, Nanjing, China) with an annealing temperature of 61 °C and an extension time of 10 s. The PCR products were recovered by agarose gel electrophoresis to obtain amplicons of the BC5 library plasmids before and after screening, which were then subjected to subsequent next-generation sequencing (NGS) analysis (GENEWIZ, Suzhou, China). ..

Next-Generation Sequencing:

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
Article Snippet: .. PCR amplification was carried out using 2 × Phanta Flash Master Mix (Vazyme, Nanjing, China) with an annealing temperature of 61 °C and an extension time of 10 s. The PCR products were recovered by agarose gel electrophoresis to obtain amplicons of the BC5 library plasmids before and after screening, which were then subjected to subsequent next-generation sequencing (NGS) analysis (GENEWIZ, Suzhou, China). ..

Touchdown PCR:

Article Title: ABRAXAS1-HELQ is a candidate class-matched reference chimeric RNA and contributes to prostate cell motility through MCOLN2
Article Snippet: Total RNA (1 μg) was reverse transcribed using the HiScript III 1st Strand cDNA Synthesis Kit with gDNA wiper (Vazyme Biotech, China) according to the manufacturer’s instructions. .. The resulting cDNA was used as a template for touchdown PCR (TD-PCR) with 2× Phanta Flash Master Mix (Dye Plus, Vazyme Biotech, China). ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Screening and Validation of qRT-PCR Reference Genes in Different Tissues and Autumn Leaf Coloration Period of Euonymus maackii
Article Snippet: .. RT-PCR reactions were conducted following the instructions of the 2× Phanta Flash Master Mix (Dye Plus) (Vazyme, Nanjing, China). ..

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